Figure 1 | Scientific Reports

Figure 1

From: Concentration polarization of ox-LDL activates autophagy and apoptosis via regulating LOX-1expression

Figure 1

Concentration polarization of ox-LDL increases LOX-1 expression and ox-LDL uptake.

(A) Schematic drawing of the experimental perfusion system. The overflow head-tank provides a steady flow to HUVECs and thoracic aorta segment. (B) Water filtration rate (vw) and (C) relative wall concentration (cw/c0) of HUVECs in permeable group. (D) and (E) Note a significantly greater increase in LOX-1 expression and DiI-ox-LDL uptake in the permeable group compared with the non-permeable group. The samples were derived from the same experiment and gels/blots were processed in parallel. HUVECs were treated with increasing concentration of ox-LDL for 24 hours under steady shear stress and constant perfusion pressure within the flow chamber kept at 1.3 Pa and 100 mmHg, respectively. Bar graphs represent data in mean ± SD based on 5 experiments, * P < 0.05 vs. non-permeable group.

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