Figure 3
DAGLα overexpression does not affect COS-7 physiology and induces 2-AG accumulation.
(a–b2) Endogenously produced DAGLα in non-transfected COS-7 (“parent”) cells is undetectable by indirect immunohistochemistry (open arrowheads; a–a2). In contrast, DAGLα, when highly expressed in COS-7 cells transfected with a DAGLα-V5 vector, accumulates in the plasma membrane (solid arrowheads; b–b2). (c) COS-7-DAGLα cells expressed DAGLα protein and (c1) synthesized 2-AG in a tetrahydrolipstatin (THL)-sensitive fashion. (d–d3) DAGLα overexpression did not influence COS-7 cell morphology (d,d1), including their surface area (d2) and density in culture (d3). Data were normalized to mean values from COS-7 “parent” cells. Abbreviations: n.s. = non significant. Scale bars = 20 μm (b,d1).
