Figure 5

Pharmacological inhibition of DAGLα or CB₁Rs prevents chemorepulsion of cholinergic growth cones (GC).

(a) Representative photomicrographs of cholinergic neurite-COS-7(-DAGLα) cell contacts upon inhibiting DAGLs (THL, O-3841) or CB₁R antagonism (AM 251, O-2050) at 3DIV. Arrows point to cholinergic neurites. Dotted lines indicate the membrane surface of COS-7(-DAGLα) cells. (b,c) Disrupting excess 2-AG signaling prevented chemorepulsion of cholinergic GCs. None of the ligands affected the angle of approach (d), neurite outgrowth (e), or cholinergic-to-COS-7 cell distance (f). Data were expressed as means ± s.e.m. except for (c) where individual data points and mean values (horizontal lines) were plotted. Data were averaged from duplicate samples from 2 independent experiments; n = 12–30 cells/group. **p < 0.01, *p < 0.05, ⁺p < 0.1. Scale bar = 20 μm (a).