Figure 6
Expression and protein differences between GPS alleles in Koshihikari and Takanari.
(a) Flag leaves in Koshihikari, Koshihikari NIL-GPS, Takanari and Takanari NIL-GPS. (b) Expression analysis by quantitative real-time PCR of NAL1 in flag leaves at three developmental stages. DBH, days before heading. Each symbol represents mean ± s.d. (n = 4); n.s., not significant between Koshihikari and Takanari (Student's t-test). (c) Western blot analysis of NAL1 protein extracted from flag leaves. Molecular weight marker and samples were run on the same gel and were electro-transferred onto the same membranes. Each column in the graph represents mean ± s.d. (n = 3). *P < 0.05 versus Koshihikari (Student's t-test).
