Figure 1
A marked increase in serum creatine (CK) levels and hyaline degeneration in the diaphragm of neonatal dystrophic dogs after initial pulmonary respiration.
(A) The serum CK levels in neonatal normal (n = 71), carrier (n = 37) and dystrophic (n = 41) dogs were compared after natural and elective Cesarean deliveries. ** p < 0.01; *** p < 0.001. (B) Serum CK levels in the cord and venous blood after initial respiration in normal (n = 5), carrier (n = 3) and dystrophic (n = 6) dogs. * p < 0.05; ** p < 0.01; *** p < 0.001. (C) Time course of changes in serum CK levels in cord and venous blood after initial respiration in normal (black; n = 5), carrier (red; n = 3) and dystrophic (blue; n = 6) dogs. * p < 0.05 dystrophic vs. normal, ** p < 0.01 dystrophic vs. normal or dystrophic vs. carrier; ## p < 0.01: dystrophic vs. carrier. (D) Hematoxylin-eosin (H&E) and alizarin red staining of diaphragms of normal and dystrophic dogs before respiration and 1 hour after respiration. Muscle stained by alizarin red indicates a high cytosolic calcium concentration. Bar indicates 100 μm.
