Figure 4 | Scientific Reports

Figure 4

From: Initial Pulmonary Respiration Causes Massive Diaphragm Damage and Hyper-CKemia in Duchenne Muscular Dystrophy Dog

Figure 4

c-fos, egr-1, IL6 and IL8 overexpressed after diaphragm damage in neonatal dystrophic dogs.

(A) Comparison of relative mRNA levels of c-fos, egr-1, IL6 and IL8 in normal (Nor) and dystrophic (Dys) dogs before (n = 4, each) and after (n = 4, each) the respiration. Bar: mean ± SD; * p < 0.05; ** p < 0.01; *** p < 0.001. (B) Western blotting of c-Fos, EGR-1, IL6 and IL8 in normal and dystrophic dogs before and after the respiration. Αctin: loading control. The blots and gels are cropped and the full-length gels and blots are included in the supplementary information. (C) H&E and immunohistochemistry of c-Fos, EGR1, IL6, IL8 (all green) and DAPI (blue) in the dystrophic diaphragm before respiration. Bar indicates 100 μm.

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