Figure 6
Microtubule disruption results in no uptake of QDs.
hMSCs were treated with 5 μg/ml of nocodazole for 4 h prior to the addition of l-MTAS-conjugated QDs. The cells were fixed and stained with QDs (a) and DAPI (blue, (b)). The disrupted microtubules can be seen with anti-tubulin antibody staining (green, (c)) and in the composite image (d). It is clear that the uptake of QDs conjugated with l-MTAS peptide is inhibited completely in the absence of organized microtubules (a). Scale bar: 50 μm.
