Figure 1

Lixiviant metabolic engineering of Chromobacterium violaceum for enhanced cyanide production and gold recovery.

Schematic of genomic region between locus tags CV_0677 and CV_0678 of wild-type and engineered C. violaceum. The duplicated hcnABC operon, with the respective pBAD or pTAC promoter, was inserted into the glmS gene using Tn7-based transposition.