Figure 3 | Scientific Reports

Figure 3

From: Molecular logistics using cytocleavable polyrotaxanes for the reactivation of enzymes delivered in living cells

Figure 3

Reduction-induced release of the β-gal from the complexes.

(A, B) Native agarose gel electrophoresis image of cleavable DMAE-SS-PRX/β-gal (N/C 2) (A) and non-cleavable DMAE-PRX/β-gal complexes (N/C 2) (B) in the presence of various concentrations of glutathione (GSH). (C) Change in relative enzymatic activity of the cleavable DMAE-SS-PRX/β-gal (N/C 2, closed circles) and non-cleavable DMAE-PRX/β-gal complexes (N/C 2, open circles) after 1 h incubation in the presence of various concentrations of GSH. Data are expressed as the means ± SD of triplicate experiments.

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