Figure 5
Enzymatic activity of the β-gal delivered into the cells.
(A-D) CLSM images of HeLa cells after 24 h incubation with β-gal (A), cell-penetrating peptide-based Xfect/β-gal (B), cleavable DMAE-SS-PRX/β-gal (N/C 2) (C) and non-cleavable DMAE-PRX/β-gal (N/C 2) (D), followed by 30 min incubation after addition of TG-β-gal as a fluorescent substrate (scale bars: 50 μm). (E) Time-course of the fluorescence intensity change of TG-β-gal in HeLa cells after 24 h incubation with β-gal (closed circles), Xfect/β-gal (open circles), cleavable DMAE-SS-PRX/β-gal (N/C 2) (closed diamonds), non-cleavable DMAE-PRX/β-gal (N/C 2) (closed triangles) and non-treated (open squares). The initial concentration of β-gal in the medium was adjusted to 20 nM. Data are expressed as the means ± SD of triplicate experiments.
