Table 3 List of plasmid constructs used in this study

pProEx-HTc

E. coli expression vector with N-terminal His₆-tag

Invitrogen

pProEx-HTc-SahH

Expression of His₆-SahH in E. coli

This study

pProEx-HTc-PknB_c

Expression of His₆-PknB_c (cytosolic domain) in E. coli

⁴⁰

pProEx-HTc-PstP_c

Expression of His₆-PstP_c (cytosolic domain) in E. coli

⁴⁰

pETDuet-1

E.coli dual expression vector containing His₆-tag in MCS-1 and S-tag in MCS-2

Novagen

pETDuet-SahH

Expression of His₆-SahH in MCS-1 in E. coli

This study

pETDuet-PknB

Expression of MBP-PknB in MCS-2 in E. coli

^{27, 39}

pSD5

Mycobacterial expression vector with Kanamycin resistance

³⁷

pSD5-SahH

Expression of His₆-SahH in M. smegmatis MC² 155

This study

pYUBDuet

Inducible shuttle vector for expression of proteins in M. smegmatis MC² 4517

³⁸

pYUBDuet-SahH

Expression of SahH under IPTG-inducible promoter in M. smegmatis MC² 4517

This study