Figure 5

Polyreactive antibodies bind to HIV-induced apoptotic cells.

HIV-infected CD4⁺ T cells were gated for apoptosis as determined by Annexin V binding. (a) Polyreactive antibodies 2E4, ZH-6 and ZH-20, but not monoreactive antibody 8512, bound to the HIV-infected apoptotic T cells. (b) Neither monoreactive nor polyreactive antibodies bound to the non-infected cells. (c) Human T cells gated by ImageStream into Annexin V⁻/gp120⁺, Annexin V⁺/gp120⁺ and double negative cells. (d) Representative images showing that Annexin V⁺/gp120⁺ apoptotic cells bind polyreactive 2E4 antibody, but not (e) monoreactive 8512 antibody.