Table 3 S-carbamidomethyl cysteine-containing gluten peptides, detected by LC-MS/MS after thermolytic digestion of a reduced/alkylated protein extract of wheat flour (cv. Akteur)
Amino acid sequence | Mr | M/z | Score | RT (min) | Present in |
|---|---|---|---|---|---|
LCCQQ | 707.3 | 708.3 | 36 | 11.8 | α-like LMW-GS, α-gliadin |
LGQC | 476.2 | 477.2 | 20 | 10.8 | m-, s- and i-type LMW-GS |
LGQCS | 563.2 | 564.2 | 16 | 11.0 | m-, s- and i-type LMW-GS, γ-like LMW-GS |
LKACQQ | 746.4 | 747.3 | 16 | 22.4 | Dx-type HMW-GS |
LNPCKN* | 744.4 | 373.3 | 18 | 11.9 | γ-like LMW-GS, γ-gliadin |
LQQCKP | 772.4 | 773.4 | 16 | 10.8 | γ-like LMW-GS, γ-gliadin |
LQQCNP | 758.3 | 759.3 | 28 | 15.5 | γ-like LMW-GS, γ-gliadin |
LQQQCIP | 885.4 | 886.5 | 32 | 30.4 | i-type LMW-GS |
LQQQCSP | 859.4 | 860.4 | 40 | 16.4 | m-, s- and i-type LMW-GS |
VRPDCST | 833.4 | 834.4 | 18 | 11.4 | α-like LMW-GS, α-gliadin |
VSAKCRS | 806.4 | 404.3 | 17 | 5.7 | γ-like LMW-GS, γ-gliadin |
VSPDCST | 764.3 | 765.3 | 23 | 14.0 | γ-like LMW-GS, γ-gliadin |
VYIPPYCT | 1011.5 | 1012.5 | 23 | 42.0 | α-like LMW-GS, α-gliadin |
VYVPPDCST | 1036.5 | 1037.5 | 32 | 28.2 | γ-like LMW-GS, γ-gliadin |
VYVPPECS | 949.4 | 950.4 | 44 | 29.2 | γ-like LMW-GS, γ-gliadin |
