Table 4 ICAT-labeled cysteine-containing peptides from bovine serum albumin, detected after a single LC-MS/MS run. Cysteine residues were labeled according to the new protocol and all peptides were manually confirmed as described in the materials and methods section
Amino acid sequence | Mr | z | M/z | Score | RT (min) |
|---|---|---|---|---|---|
AEDKDVCKNY | Light: 1410.6 | 2+ | 706.3 | 97 | 20.8 |
| Â | Heavy: 1419.6 | Â | 710.8 | 37 | 20.8 |
| Â | Â | 3+ | 471.3 | 69 | 20.9 |
| Â | Â | Â | 474.3 | 15 | 20.8 |
CDEF | Light: 739.3 | 1+ | 740.2 | 20 | 29.2 |
| Â | Heavy: 748.3 | Â | 749.3 | 38 | 29.2 |
ECADDRADL | Light: 1233.5 | 2+ | 617.7 | 16 | 26.2 |
| Â | Heavy: 1242.5 | Â | 622.3 | 28 | 26.3 |
| Â | Â | 3+ | 412.3 | 15 | 26.2 |
| Â | Â | Â | 415.3 | 15 | 26.1 |
