Figure 3

hMSC downregulated NKT-like cells and increase number of T regulatory cells.

(a) There was significant decrease in percentage and total number (b) of CD3⁺NKp46⁺ NKT-like cells in spleens of tumor-bearing mice that received hMSC. Isotype controls (c) and representative dotplots (d) confirm significant decrease in percentage of NKT cells in spleens of hMSC treated mice. Combined results of 4 experiments with at least 9 animals per group (mean ± s.e.m.; *P < 0.05). (e) There was significant increase in both percentage and total number (f) of Foxp3⁺ regulatory cells cells in spleens of tumor-bearing mice that received hMSC. Representative histogram (g) confirms significant increase in percentage of Foxp3⁺ T regulatory cells in spleens of hMSC treated mice. Histograms and mean values presented in the graphs are gated on CD4⁺ cells. Combined results of 4 experiments with at least 9 animals per group (mean ± s.e.m.; *P < 0.05).