Figure 3

(a) The Raman spectra of S. aureus and P. aeruginosa obtained by a smooth Au substrate at high bacteria concentration by using the coffee-ring technique, which are much weaker than the ACEK-SERS spectra obtained by the roughened Au surface using ACEK concentration. (b) The bacteria are randomly distributed when no voltage is applied to the electrodes. (c) Result of a concentration of 3 × 104 CFU/ml bacteria suspended in a 50 μl buffer with an AC voltage at 800 Hz for 3 minutes. (d) Result of an ultra low bacteria concentration of 5 × 103 CFU/ml sample under the same condition.