Figure 1

Susceptibility of tumor cells to VSV infection in vitro and in vivo.

(a) Propagation of VSV-GFP was quantitated by TCID₅₀ titrations and fold increase in virus yield (virus output/input) is calculated (n = 3 replicates, mean ± SD). (b) Cell viability in VSV infected cultures was evaluated by the MTS assay at 48 h after infection at the respective MOIs. (c) Representative image of immunohistochemical staining for VSV protein (Alexa-555/red staining) in MPC-11, LM-1 and EMT-6 xenografts at 24 h post intravenous VSV delivery. (d) Red fluorescent 200 nm polystyrene microspheres in the tumors at 2 h after intravenous delivery. (e) Quantitative RT-PCR for VSV nucleocapsid mRNA in the tumors at 2 h or 24 h post virus delivery. Mean ± SD (n = 3 mice per time point). * P ≤ 0.05. Unpaired student t test was used. (f) Abundant and uniform distribution of CD68 cells (Alexa488/green staining) in the tumors. Scale bar represents 100 μm.