Figure 4
From: Induction of antiviral genes by the tumor microenvironment confers resistance to virotherapy
The effect of JAK inhibitors on the macrophage induced anti-viral state and relative sensitivity of cancer cell lines to type I IFN.
(a) ISRE-Luc expressing LM-1 and (b) EMT-6 cells were exposed to RAW264.7 conditioned media in the presence of ruxolitinib for 24 hours, after which the luciferase activity was measured (ISRE induction) or the cells were infected with VSV-GFP (MOI 1.0) and cell viability was determined 48 h later. Results represent mean ± SD of three different experiments. *P ≤ 0.05. Unpaired student t test was used. (c) Sensitivity of cancer cell lines to type I IFN was tested by pretreating the cells with the indicated concentrations of mouse IFNα for 24 h before VSV-GFP infection (MOI 1.0). Cell viability is presented as a percentage of the uninfected control.
