Figure 1
From: Identification and super-resolution imaging of ligand-activated receptor dimers in live cells
Live cell super-resolution imaging of functional membrane EGFRs newly activated by their ligand.
(a) Principle of the super-resolution method. Oblique illumination (light green) does not excite EGF ligands in solution. (b) uPAINT image of EGFR labeled by EGF-Atto532 acquired on live cells. (c) Same experiment performed using Panitumumab-Atto647N. (d) Competition assay showing specificity of EGF-Atto532 labeling: number of fluorescent EGF detected per frame (50 ms) on the cell membrane during a uPAINT acquisition using EGF-Atto532. After ~8 s and 38 s (red arrows), unlabeled Panitumumab was added in 100-fold excess (40 nM) compared to EGF.
