Figure 2
From: Identification and super-resolution imaging of ligand-activated receptor dimers in live cells
Live cell super-resolution imaging of membrane EGFR dimers based on single-molecule FRET.
Dual color uPAINT imaging of EGFR was performed using a 1:1 mix of EGF-Atto532 and EGF-Cy5 under 532 nm laser excitation. (a) Schematics of single molecule FRET between two fluorescent ligands bound on a EGFR dimer. (b) Donor channel: super-resolved image of EGFR labeled by EGF-Atto532 as in Fig. 1b. (c) Acceptor channel: super-resolved image of EGF activated dimer EGFRs obtained by single molecule FRET. (d) Signature of single molecule FRET: anti-correlated fluorescence signals detected by single molecule fitting in the donor (green line) and acceptor (red line) channels, in corresponding positions. Insets in (b) and (c) represents zooms of highlighted regions showing preferential cell edge localization of the dimers.
