Figure 2
From: Microfluidic isolation of highly pure embryonic stem cells using feeder-separated co-culture system
Proliferation and viabilities of mEFs and mES cells on the co-culture microdevice.
(A, B) The fluorescence profile of RFP-mEFs at day 1 and 5 showing the cell proliferation, scale bar: 100 μm. (C) The curve of fluorescence intensity folds from day 1 to day 7. (D, E. F) The viabilities of inactivated mEFs and P3 mEFs were characterized with Calcein AM/EthD-1 (10 μL, 10 mg/mL) at day 5 and imaged by the fluorescence microscopy. (G) Confocal morphology of RFP-mEFs/mES cells, stained with Hoechst 33342. (H, I) Viabilities of P3 mEFs/mES cells and inactivated mEFs/mES cells on 3D-device, stained with cell live/dead kit (Calcein AM/EthD-1). Scale bar: 100 μm.
