Figure 3

Improving the methylacetoin biosynthesis from acetoin by coupling the conversion to acetaldehyde dehydrogenation.

Acetoin and acetone were incubated with the AcoAB enzyme sample (left) or with the AcoAB and AcoD enzymes with the NAD⁺ cofactor (right). The reaction mixture was composed of 400 mM Tris-HCl buffer (pH 8.0), 2 mM MgSO₄, 100 mM KCl, 0.2 mM ThDP, 1 mM thioglycol, 2.5 mM NAD⁺, 50 mM acetone and 1 mM acetoin. The reactions were initiated by adding the enzymes (time = 0) and were conducted at 30°C for 12 h to reach equilibrium. The reaction mixtures were analyzed by GC. I.S., internal standard (1-butanol).