Figure 1
Apigenin inhibits miRNA function.
(a), Apigenin inhibits endogenous miRNA function. Huh7 cells were transfected with reporters to determine the functions of the indicated miRNAs. Twenty-four hours after treatment with the indicated substances, reporter assays were performed. Data represent the means ± standard deviation (s.d.) from three independent experiments. *, p < 0.05 (t-test). (b), Apigenin inhibits the function of exogenously overexpressed miRNAs. Huh7 cells were transfected with reporters and corresponding miRNA precursor-expressing plasmids or an empty vector. Twenty-four hours after treatment with the indicated substances, reporter assays were performed. Data represent the means ± s.d. from three independent experiments. *, p < 0.05 (t-test). (c), Dose-dependent effects of apigenin on miRNA function. Huh7 cells were transfected with reporter plasmids to determine miR122 function. Cells were treated with indicated doses of apigenin for 24 h and luciferase assays were performed. Caffeine was included as a negative control. Data represent the means ± s.d. from three independent experiments. *, p < 0.05 (t-test) compared with the negative control.
