Figure 1

Characterization of the molecular size of KillerRed variants.
(a) Native PAGE analysis of KillerRed-L160T/F162T, KillerRed and the YFP variant Venus. (b) SDS-PAGE analysis of KillerRed and SuperNova. (c) Gel filtration chromatography of SuperNova (red line), KillerRed (blue line), mSEGFP (green line) and molecular weight marker proteins (gray line) through a Superdex75 100/300GL column. Proteins were detected by their absorption at 280 nm.