Figure 2 | Scientific Reports

Figure 2

From: SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

Figure 2

Results of analytical ultracentrifugation (AUS) analyses for SuperNova, mCherry and KillerRed.

(a) AUC sedimentation velocity (AUC-SV) analysis at loading a concentration of 100 μM. Plot of the distribution of sedimentation coefficients (C (s) versus s, where s is plotted in Svedberg units, (S) calculated from AUC sedimentation velocity experiments. Majority of SuperNova, mCherry and KillerRed were 3.1 S, 3.0 S and 4.7 S, respectively. (b–d) Concentration gradient of AUC sedimentation equilibrium experiments at 20,000 r.p.m. Data sets (black) were fit to the monomeric (green) and dimeric (red) molecular weights. Determined average molecular weights (Mw) were 29.8 kDa, 31.5 kDa and 60.2 kDa for (b) SuperNova, (c) mCherry and (d) KillerRed, respectively.

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