Table 2 The products of batch bioconversion with different cofactor regeneration systems

From: Engineering of cofactor regeneration enhances (2S,3S)-2,3-butanediol production from diacetyl

Strain

(2S,3S)-2,3-BD (g/L)

AC (g/L)

Acetic acid (g/L)

Lactic acid (g/L)

Gluconic acid (g/L)

Productivity of (2S,3S)-2,3-BD (g/(L·h))

Yield of (2S,3S)-2,3-BD (%)

E. coli BL21 (DE3) (pETDuet-bdh)

16.1 ± 0.30

2.4 ± 0.08

7.3 ± 0.21

11.6 ± 0.21

ND

2.7 ± 0.05

82.5 ± 0.62

E. coli BL21 (DE3) (pETDuet-bdhgdh)

16.8 ± 0.49

1.9 ± 0.26

6.6 ± 0.21

6.2 ± 0.13

5.2 ± 0.20

2.8 ± 0.08

85.4 ± 1.59

E. coli BL21 (DE3) (pETDuet-bdhfdh)

17.8 ± 0.59

1.1 ± 0.28

ND

ND

ND

3.6 ± 0.12

91.8 ± 1.18

  1. ND: not detected.
  2. Yield of (2S,3S)-2,3-BD: the (2S,3S)-2,3-BD produced divided by the DA consumed and multiplied by 100.
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