Table 3 Strains, plasmids and primers used in this study
From: Engineering of cofactor regeneration enhances (2S,3S)-2,3-butanediol production from diacetyl
Strain, plasmid or primer | Genotype, property or sequence | Source or reference |
|---|---|---|
Strain | ||
B. subtilis 168 | Wild type | ATCC 23857 |
C. boidinii | Wild type | NCYC 1513 |
E. coli DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Novagen |
E. coli BL21 (DE3) | F− ompT hsdSB (rB−mB−) gal (λ c I 857 ind1 Sam7 nin5 lacUV5 T7gene1) dcm (DE3) | Novagen |
Plasmid | ||
pEASY-Blunt | Cloning vector; Apr | TransGen |
pETDuet-1 | Overexpression vector; Apr | Novagen |
pETDuet-bdh | bdh in pETDuet-1 | |
pETDuet-gdh | gdh in pETDuet-1 | This work |
pETDuet-bdhgdh | bdh and gdh in pETDuet-1 | This work |
pETDuet-bdhfdh | bdh and fdh in pETDuet-1 | This work |
Primer | ||
pg1 | 5′-CCCAGATCTAATGTATCCGGATTTAAAAGG-3′ | This work |
pg2 | 5′-CTCGAGTTAACCGCGGCCTGCCT-3′ | This work |
pf1 | 5′-CATATGAAGATCGTTTTAGTCTTATATGATGCTGGTA-3′ | This work |
pf2 | 5′-CTCGAGTTATTTCTTATCGTGTTTACCGTAAGCTTTG-3′ | This work |
