Figure 1
From: Anti-LRP/LR specific antibodies and shRNAs impede amyloid beta shedding in Alzheimer's disease
Co-localisation of LRP/LR with the AD relevant proteins APP, β- and γ-secretase on the cell surace.
Cell surface proteins on HEK293 cells were indirectly immunolabelled to allow for detection using the Olympus IX71 Immunofluorescence Microscope and Analysis Get It Research Software. (a) APP, (e) β-secretase, (i) γ-secretase and (m) VLA6 were all indirectly labelled with Alexafluor 633, while an anti-human FITC conjugated antibody was used to label IgG1-iS18 bound to LRP/LR (b, f, j, n). The merged images between LRP/LR and relevant proteins are shown (c, g, k, o) and the corresponding 2D-cytofluorograms have been included to confirm the degree of co-localisation (d, h, l, p).
