Figure 2
Tyrosine phosphorylated β-dystroglycan is in the nucleus of prostate epithelium in vivo.
Immunohistochemistry of paraffin embedded TMA of prostate stained for pY890 β-dystroglycan shows a clear presence of tyrosine phosphorylated β-dystroglycan in the nucleus of prostate epithelium ((A–E) and arrows in magnified regions), scale bar is 100 um and in inset is 50 um. Cellular fractionation of prostate tissue also revealed the presence of both non-phosphorylated β-dystroglycan (F) or tyrosine phosphorylated β-dystroglycan (G) in nuclear fractions from either benign or tumour tissue. For non-phosphorylated β-dystroglycan the full length 43 kDa and cytoplasmic 26 kDa were the predominant species found in the nuclear fraction, whereas for phosphorylated β-dystroglycan the 31 kDa transmembrane and cytoplasmic fragment was more prominent in the nucleus. Fibrillarin and tubulin are shown as a nuclear (N) and cytoplasmic (C) fraction markers respectively. Both tumour samples were from hormone refractory primary TURPs.
