Figure 4
From: Inhibition of the hypoxia-inducible factor pathway by a G-quadruplex binding small molecule
CL67 inhibited the expression of HIF-1α and HIF-1 regulated genes.
(a), (b) and (d)Total RNA was purified from RCC4 cells exposed to 5× the IC50 of CL67 for 2, 4 or 6 hours, reverse transcribed to cDNA and then used to monitor the expression changes of 83 genes involved in angiogenesis by qPCR. Expression data for each gene was normalised to the housekeeping gene RPLP0 and then to untreated samples at t0 (n = 3; error bars, 90% confidence interval; *, p < 0.05 and **, p < 0.01 compared with untreated control using Student's t-test). (c) HIF-1 regulated angiogenesis pathway genes were significantly down-regulated by CL67 compared to HIF-1 independent genes. Shown are box and whisker plots of log2 fold expression changes for 59 genes that are not controlled by the HIF-1 pathway (Non-HIF-1) and 15 genes that are established targets of HIF-1 (HIF-1). Significance values are for 2-tailed, homoscedastic t-tests comparing non-HIF-1 and HIF-1 regulated genes at each time point.
