Figure 1
From: Metabolic Characterization of a Sirt5 deficient mouse model
Generation and validation of a Sirt5 deficient mouse model.
(a). Targeting strategy for disruption of the mouse Sirt5 gene. LoxP sites were inserted to flank exon 4 of the Sirt5 gene. The resulting Sirt5floxed mice were bred with CMV-Cre transgenic mice to generate Sirt5−/− mice. (b). Sirt5 mRNA levels in selected tissues of Sirt5+/+ and Sirt5−/− mice. Three pairs of 8-week-old wild-wt(wt) and knockout male mice were used for the validation. (c). Sirt5 protein levels in liver and lung of Sirt5+/+ and Sirt5−/− mice. Two pairs of 8-week-old wt and knockout male mice were used for protein analysis. (d). The birth and sex ratio of Sirt5+/+, Sirt5+/−, Sirt5−/− mice. 328 mice were genotyped and analyzed. (e). Global protein succinylation in liver and gastrocnemius muscle. 3 pairs of 12-week-old wt and knockout male mice were fasted for 24 hours and sacrificed for protein analysis. (f). Serum ammonia levels. 12-week-old Sirt5+/+ and Sirt5−/− male mice were fasted for 24 hours and sacrificed. Blood was collected by cardiac puncture. Serum was immediately frozen in liquid nitrogen and used for measurement of ammonia levels. N = 4. * P< 0.05.
