Figure 3
Use of the ERF4 distal polyadenylation site is increased in fpa plants.
(A) Distribution of direct RNA sequencing (DRS) normalized reads at the ERF4 locus. DRS was performed on RNAs from 14-day-old seedlings of Col-0 and fca-9, fpa-7 and fpa-7 fca-9 flowering time mutants for the first experiment and of Col-0 and the fld-3 flowering time mutant for the second experiment. Representative Col-0 data for each experiment is shown above the mutant data. Exons are denoted by rectangles and UTRs by adjoining narrower rectangles. (B) Proposed designation of alternating U- and A-rich sequences at the ERF4-A or ERF4-IR downstream cleavage sites. Location of the AAUAAA and AUGUUU cis elements (AUGUUU corresponds to the point mutation in the UUGUUU motif) are displayed, as are their positions relative to the cleavage site. USE, upstream sequence element; PAS, polyA signal; Fip1, the U-rich sequence upstream of the cleavage site is the proposed Fip1-binding site56,57; DSE, downstream sequence element; black triangle, cleavage site. (C) Expression of the ERF4 amplicons indicated in Fig. 2A in Col-0, 35S::FPA, fpa-7 and fpa-8. Data represent the mean and standard error of three biological replicates. The asterisk indicates a difference from Col-0 (P < 0.05).
