Figure 5 | Scientific Reports

Figure 5

From: The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation

Figure 5

ERF4 isoforms differentially regulate both flg22-triggered ROS burst and PDF1.2 expression.

(A) RT-PCR showing the presence or absence of ERF4 isoforms 30 min after 1 μM flg22 treatment of Col-0, erf4, erf4/ERF4-A and erf4/ERF4-R seedlings. (B) The oxidative burst elicited by 1 μM flg22 in representative ERF4 isoform complementation (left) and overexpression (right) lines. Data represent the mean and standard error of three biological replicates. (C). Expression of PDF1.2 in 14-day-old seedlings of the indicated genotypes. Data represent the mean and standard error of three biological replicates.

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