Figure 6 | Scientific Reports

Figure 6

From: The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation

Figure 6

FPA inhibits flg22-triggered induction of ERF4-A.

(A) Expression of the ERF4 amplicons indicated in Fig. 2B in Col-0, fls2, 35S::FPA, fpa-7 and fpa-8 seedlings either untreated (left) or treated with 1 μM flg22 for 1 h (right). Data represent the mean and standard error of three biological replicates. The asterisk indicates difference from Col-0 (P < 0.05). (B) Schematic model indicating the role of flg22 and FPA in ERF4 processing. FPA promotes use of the proximal poly(A) site, inhibiting readthrough to the canonical distal poly(A) site. flg22 promotes distal poly(A) site use, possibly by inhibiting proximal poly(A) site use, allowing readthrough and subsequent splicing.

Back to article page