Figure 5
From: Recombinant Human Prion Protein Inhibits Prion Propagation in vitro
Inhibition of PrPSc propagation by recombinant human PrP23-231 in ScN2a cells.
Different amounts of rHuPrP23-231 ranging from 0 to 1 μM were added into the cell culture medium for four days. The cells were lysed and subjected to PK-digestion at 25 μg/ml prior to SDS-PAGE and Western blotting with 6D11. The intensity of PK-resistant PrPSc was significantly decreased at 0.1 μM of rHuPrP23-231 or greater. β-actin was determined to normalize the levels of individual samples examined. The blot is a representative of three independent experiments.
