Figure 1 | Scientific Reports

Figure 1

From: Conformational change of Sos-derived proline-rich peptide upon binding Grb2 N-terminal SH3 domain probed by NMR

Figure 1

(A) 1H-13C HSQC spectra of 13C/ 15N-labeled VPP peptide. Free (blue) and Grb2 nSH3-bound (red) peptide spectra are overlaid. Cross-peaks are labelled with one-letter amino acid codes and the residue number. The minor peaks derived from the cis Xaa–Pro bond are also labelled. (B) Chemical shift differences between the free and Grb2 nSH3-bound states plotted as functions of amino acid sequence for the VPP peptide, where Δppm is defined as (0.5*[0.3*(ΔωC)2 + (ΔωH)2])1/2. (C) Schematic model of the binding of the VPP peptide to Grb2 nSH3.

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