Table 1 Chemical shifts (ppm) of the main chain atoms for the VPP peptide in the free and Grb2 nSH3-bound states
free state | Grb2 nSH3 bound state | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Residue | 15N | 13Cα | 1Hα | 13C′ | 15N | 13Cα | 1Hα | 13C′ | |Δ Cα|* |
V1 | 122.4 | 59.7 | 4.48 | 174.1 | 122.7 | 59.3 | 4.16 | 173.4 | 0.4 |
P2 | 141.5 | 61.5 | 4.71 | 174.1 | 140.6 | 61.0 | 3.57 | 174.0 | 0.5 |
P3 | 136.0 | 61.3 | 4.74 | 174.8 | 134.7 | 61.2 | 4.54 | 173.3 | 0.1 |
P4 | 134.9 | 62.8 | 4.47 | 176.7 | 135.0 | 62.6 | 4.42 | 176.7 | 0.2 |
V5 | 121.0 | 59.7 | 4.42 | 174.3 | 124.3 | 60.5 | 4.22 | 174.4 | 0.8 |
P6 | 141.2 | 61.5 | 4.70 | 174.8 | 142.6 | 61.4 | 4.84 | 174.3 | 0.1 |
P7 | 134.9 | 62.8 | 4.45 | 176.8 | 133.7 | 62.8 | 4.58 | 177.5 | 0 |
R8 | 120.6 | 56.0 | 4.32 | 176.2 | 122.1 | 55.4 | 4.15 | 176.0 | 0.6 |
R9 | 122.7 | 56.0 | 4.37 | 175.2 | 123.8 | 56.1 | 4.24 | 175.1 | 0.1 |
R10 | 127.1 | 57.4 | 4.18 | 180.9 | 128.2 | 57.3 | 4.07 | 180.9 | 0.1 |
