Figure 2
Analysis of osmotic stress tolerance in the T1 transgenic lines.
(a) Leaf disk senescence assay for osmotic tolerance in transgenic tobacco plants (L12.1, L12.2 and L12.3) in 200 mM NaCl. Transgenic lines showing high osmotic stress tolerance as compared to controls (WT and VC). (b) Remaining chlorophyll content from leaf disks of WT, VC plants and the transgenic lines after incubation 200 mM NaCl solution (Clh A and Clh B are shown in dark red and light green bar respectively). Disks floated in water served as the experimental control (yellow bar represents total chlorophyll content in the control condition). (c) Germination and seedling growth of WT, VC and T1 transgenic lines (L12.1, L12.2 and L12.3) under water condition (for control) and 200 mM NaCl (for experiment). (d) Phenotypic comparison of growth of T1 transgenic tobacco plants overexpressing PiCypA gene (L12.1, L12.2 and L12.3) with controls plants (WT and VC) in the presence of water and 200 mM NaCl. Seeds were germinated for 7 days and plantlets grown on water (upper row) and on 200 mM NaCl (lower row). (e) WT, VC and transgenic plants (L12.1, L12.2 and L12.3) in soil pots supplied with 200 mM NaCl solution. Note that the WT and VC plants could not sustain growth under osmotic stress. (f) Phenotypic comparison of growth of T2 transgenic tobacco plants overexpressing PiCypA gene (L12.1.1, L12.2.1 and L12.3.1) with control plants (WT and VC) in the presence of water and 200 mM NaCl.
