Figure 3 | Scientific Reports

Figure 3

From: Isolation and characterization of a novel wheat cysteine-rich receptor-like kinase gene induced by Rhizoctonia cerealis

Figure 3

Phylogenetic analysis of the deduced amino acid sequences of TaCRK1 and 19 additional RLKs and comparison of CRR motif sequences.

(a) Phylogenetic tree constructed by neighbor-joining algorithms of MEGA 5.0 software after the multiple RLK protein sequences alignment using the CLUSTAL W program. Bootstrapping was performed 1,000 times to obtain support values for each branch. Four groups of RLK proteins, including cysteine-rich RLK (CRK), Lectin RLK (LecRK), LysM RLK (CERK) and LRR RLK, were represented by letters A, B, C and D, respectively. The GenBank accession numbers of RLK protein sequences are as follows: AtCRK13 (AEE84724), ZmCRK (AFW74556), OsCRK (BAC65053), BdCRK (XP_003560118), TaRLK10 (AAC49629), TaRLK3 (AAQ82627), TaRLK2 (ABB84341), TaRLK1 (ABB84340), AtLecRK (AEE79957), OsLecRK (AAT77694), TaLecRK (ACN41357), AtCERK1 (BAF92788), OsCERK1 (D7UPN3), AtBAK1 (AEE86224), HvBAK1 (AEE44134), TaBAK1 (ACD49737), AtEFR (AED92850), AtFLS2 (AED95370) and OsXa21 (AAC80225). (b) Amino acid alignment of CRR motifs between TaCRK1 and CRK proteins. Boxes in red represent 100% similarity, blue for 75% similarity and yellow for 50% conserved amino acids.

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