Figure 4
Plasma membrane localization of TaCRK1-GFP fusion protein.
(a) Subcellular localization of the fused p35:TaCRK1-GFP in onion epidermal cells. The p35:TaCRK1-GFP and p35:GFP constructs were separately introduced into onion epidermal cells by bombardment and expressed under control of CaMV 35S promoter. Bars = 100 μM. (b) Subcellular localization of the fused p35:TaCRK1-GFP in wheat protoplasts. The p35:TaCRK1-GFP and p35:GFP constructs were separately introduced and expressed transiently in wheat protoplasts. Bars = 10 μM. (c) Co-localization of the fused p35:TaCRK1-GFP and CFP-labeled plasma membrane marker AtPIP2A in wheat protoplasts. The TaCRK1-GFP and AtPIP2A were co-transformed into wheat protoplasts and co-expressed under control of CaMV35S promoter. Bars = 10 μM.
