Figure 6
Effect of TaCRK1 silencing on the resistance response of CI12633 to necrotrophic pathogen Rhizoctonia cerealis.
(a) Relative transcript levels of TaCRK1 and BSMV CP genes in the 4th leaves of the mock plants or those infected by BSMV:GFP and BSMV:TaCRK1 as evaluated by semi-quantitative RT-PCR using gene-specific primers. Amplification of the wheat TaEF-1a gene served as internal control. (b) Relative transcript levels of TaCRK1 in the 4th leaves of the mock plants or those infected by BSMV:GFP and BSMV:TaCRK1 as evaluated by qRT-PCR. Relative expression of TaCRK1 indicated the changing fold of the gene transcript over plants with mock-treatment. Three biological replicates were averaged with standard error of mean indicated. Asterisks indicate statistically significant variation calculated using Student's t-test (**P<0.01). (c) Response of the 4th sheaths of the mock and BSMV virus-inoculated CI12633 and positive control Wenmai 6 to R. cerealis. The photographs were taken 2 weeks after R. cerealis inoculation. Gels in Fig. 6 were cropped and full-length gels are presented in the Supplementary Fig. 3, 4 and 5.
