Figure 1

Optical and electron microscopic evaluation of changes in cellular morphology.

(A) Phase-contrast, (B) scanning electron microscopy (red arrows indicate cell fragments) and (C) transmission electron microscopic evaluation of mitochondrial changes. Note in the TEM images that there is an increased loss in mitochondrial integrity in the PHB^– group compared with the H-R group (yellow arrows) compared with normoxic control cells (white arrows). The damage to mitochondria in the PHB⁺ was alleviated compared with the H-R group (white arrow). Red arrows indicate differences between sharp and contrast-rich membranes and the blebbed and diffuse membranes in the H-R group. H-R group: cell injury induced by hypoxia reoxygenation; H-R PHB^–: group lentivirus carrying siRNA PHB; H-R PHB⁺ group: lentivirus carrying Phb.