Figure 1
From: Targeting human embryonic stem cells with quantum dot-conjugated phages
Experimental scheme.
(A) Phages that specifically bind to human ESCs were enriched in the phage pool by two rounds of bio-panning. Each bio-panning round included two subtraction steps with dESCs, two subtraction steps with CF-1 cells and a selection step with ESCs. Monoclonal phages were enriched, purified and re-screened by enzyme linked immunosorbent assay (ELISA). Phages that showed high binding affinity and specificity were further confirmed by immunofluorescence microscopy and ELISA. The selected phages were then chemically linked to QDs and tested for hESC targeting. (B) Illustration of chemical conjugation between the phage and QDs. (C) This enlarged view of (B) shows how the –NH2 groups on the phage are conjugated to the free –COOH groups on the surface of the QDs via EDC.
