Figure 4
From: Targeting human embryonic stem cells with quantum dot-conjugated phages
Competitive binding assay with the H166 and H178 phage and free peptide.
The chemically synthesized H166 (HGAAWGTRTGHV) and H178 (HGAAWGTRTGHV) peptides were used in a competitive binding assay with the H166 phage and the H178 phage. The phages were added to hESCs, followed by the peptides at concentrations ranging from 10 nM to 10,000 nM. THAGHAGVGRWT and DTSAGVLSWGEP peptides were used as the control. The binding ratio was calculated by dividing the number of phages recovered in the presence of the peptide by the number of phages recovered in the absence of peptide. The values are shown as the mean ± standard deviation.
