Figure 6

Wild type and mutant Sry gene expression detected by western blotting.

(A) Schematic representation of constructed gene structures in the expression vector. Position of the two nucleotide insertion (TT) is shown above the mutant gene structure. (B) Detection of wt and mutant SRY protein containing FLAG and S-tag at N- and C-termini, respectively, by western blotting using anti-FLAG antibody (arrow). Lane 1: empty vector transfected cell lysate; lane 2: wt Sry with FLAG and S-tag; lane3: mutant Sry with FLAG and S-tag. Size marker is shown at left of image. (C) Detection of wt and mutant SRY protein containing FLAG and S-tag at N- and C-termini, respectively, by western blotting using anti-S-tag antibody (arrow). Lanes and markers are same as those of (B). (D) Detection of tubulin by western blotting (arrowhead) as a control for amount of protein loading. Lanes and markers are same as those of (B).