Figure 3
The effects of BM extracts on fasting plasma and liver FGF21 levels as well as FGF21 signaling in mice.
FGF21 was measured using a mouse FGF21 ELISA kit from R & D Systems Inc (Minneapolis, MN). (A) Fasting plasma FGF21 concentrations. (B) Liver FGF21 content. Mean ± SEM (n = 10/group). (C) FGF21 signaling proteins were measured by Western blotting assay. Results were normalized by β-actin content. BM-V significantly increased FGFR1, FGFR3, FGFR4 and PGC-1α, slightly reduced PPARα, but did not affect b-Klotho protein abundance in comparison with HFD animals. Mean ± SEM (n = 10/group). * P < 0.05, ** P < 0.01 and *** P < 0.001, BM-V group vs. HFD group. # P < 0.05, glucose concentrations at week 6 or week 12 vs. week 0 in HFD animals. The blots in this figure are cropped. The full length blots are supplied in the supplementary information.
