Figure 1
From: Regulation of epithelial plasticity by miR-424 and miR-200 in a new prostate cancer metastasis model
Selection of DU145 human prostate cancer cells with increased metastatic potential.
(A) Schematic of the experimental approach. DU145 prostate cells were injected orthotopically into the prostate. Lymph nodes were removed and cultured and selected tumor cells subjected to repeated rounds of orthotopic injection. Illustrations by Kristin Johnson (Vascular Biology Program, Boston Children's Hospital). (B) Photography of gross specimens (tumors and sentinel lymph nodes). DU145 parental cells and DU145-LN sublines (DU145-LN4 shown) were reinjected into the prostate and the prostate and lymph nodes were removed after 5 wks. Scale bar = 1 cm. (C) Representative H&E staining of lymph nodes from mice bearing orthotopic parental DU145 tumors (left panel, P) and DU145-LN4 tumors (center and right panels, LN4). Metastatic nodule indicated by arrowhead in center panel, magnification shown in right panel.
