Figure 3
From: Regulation of epithelial plasticity by miR-424 and miR-200 in a new prostate cancer metastasis model
In vivo prostate-cycled DU145 cells do not display the phenotypic and expression changes observed in the metastatic DU145-LN cell lines.
DU145 cells were injected into the mouse prostate, the prostate excised and grown in culture to create DU145-PR1. This process was repeated to create DU145-PR2 and DU145-PR3. (A) Photography of DU145-PR3 gross specimens (tumors and sentinel lymph nodes) after 5 weeks following cell line injection orthotopically into the prostate. Scale bar = 1 cm (B) Phase contrast microscopy shows similar cell phenotype of DU145 parental and DU145-PR3 cells in culture. (C) Western blot analysis of DU145-PR3 and parental DU145 cell lysates indicate that expression of E-cadherin, EpCAM, γ-catenin, vimentin and β-catenin were not changed by in vivo cycling through the prostate, in contrast to the expression changes observed in DU145-LN4 cells. Equal loading shown by actin immunoblots. The same membrane was stripped and reprobed for E-cadherin, EpCAM, γ-catenin, vimentin and actin. A separate blot was probed for β-catenin and actin. Images were cropped to save space; original images are shown in Supplemental Figure 5. Handwritten pen marks denote molecular weight markers.
