Figure 3
From: Enhanced transcription and translation in clay hydrogel and implications for early life evolution
Transcription and translation in the clay hydrogel environment.
(a) Effect of clay concentration on active Rluc protein expression, with fixed plasmid concentration of 20 ng/μl. (b) Effect of gene concentration on active Rluc protein expression of clay hydrogel (red triangles) over SPS controls (black squares), with fixed clay concentration of 4 μg/μl. (c) Time course of active Rluc protein expression produced from clay hydrogel (red triangles) and SPS (black squares). (d) Time course of mRNA expression from clay hydrogel (red triangles) and SPS (black squares). Error bars represent standard deviations from three measurements. (e) SDS-polyacrylamide gel electrophoresis assays of Rluc proteins. Lane 1 corresponds to the pre-stained protein standard (SeeBlue Plus2 Invitrogen, unit is kDa). Pure standard Rluc is in Lane 2 (with molecular weight of 36,113 Dalton). Lane 3 is the lysate control. Lane 4, 5 are Rluc proteins expressed from clay hydrogel and SPS, respectively (molecular weight was 35,991 Dalton). The Rluc bands in Lane 4 and 5 migrated slightly faster than Lane 2 due to slightly lower molecular weight. (f) Comparison of luminescence images of active Rluc protein expressed from clay hydrogel and SPS. Blank is the lysate control.
