Figure 1

Localization of Mincle in brain tissue after ischemic stroke in mice and humans by double-immunostaining of Mincle and marker proteins.

(A) The expression levels of Mincle and Iba1 were upregulated after ischemia. Scale bar = 20 μm. (B) Mincle co-localized with CD11b, but not Iba1, all of which are markers of immune cells. Mincle co-localized with neuronal cell marker MAP2. Mincle co-localized with the endothelial cell marker Claudin5. Mincle co-localized with Syk, which is the downstream signal of Mincle. Scale bar = 10 μm. (C) The expression levels of Mincle and Iba1 were upregulated in human stroke patients. Scale bar = 20 μm. (D) Mincle co-localized with CD11b, MAP2 and Claudin 5 in human stroke patients. Unlike in mice, Mincle also co-localized with Iba1. Scale bar = 10 μm.